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The revolution in the Bio-pharmaceutical drug discovery arena has brought out the importance of Molecular biology. With its long experience in this area since 1994, Transgene is experienced in providing its services in areas such as recombinant DNA constructs and the development of recombinant drugs. |
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Transgene can help you in developing bio-pharmaceutical drugs from the initial stage of cloning your gene of interest, through to the development of recombinant DNA constructs and finally a recombinant clone expressing the protein of interest. We provide a comprehensive suite of services to move your project along quickly and smoothly. |
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Transgene can provide the following services: |
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Gene synthesis |
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Sub cloning and plasmid construction |
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Vector construction and modification |
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Construct generation |
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Genetic (cDNA) cloning |
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In vitro transcription |
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Protein expression |
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Plasmid preparation |
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Lab scale fermentation |
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| At Transgene, we provide the following fermentation services too: |
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Scale-up of E. coli in 10-L/55 L Fermentors |
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Scale-up of yeasts in 20 L Fermentors |
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Mammalian expression in bioreactors |
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Optimization of expression in bioreactors |
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Process development for fermentation |
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Batch, fed-batch, and continuous culture |
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Intracellular and secreted products |
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At Transgene, the scientists will demonstrate each step, from the stage of cell banking and cell growth optimization in a Fermentor all the way through to the stage of protein purification, covering the various upstream and downstream processes involved. |
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Purification of recombinant proteins |
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Sequential and appropriate downstream protein purification process is extremely important in the development of any bio-pharmaceutical drug. The ultimate goal is to achieve a high degree of purity and efficacy of the targeted recombinant protein, and this process needs to be reproducible in order for the developed drug to be commercial relevant. |
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The scientists at Transgene have the expertise and long experience necessary to generate high-quality recombinant protein drugs. |
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We have expertise and experience in molecular biology and will perform many other services such as: |
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Gene knockouts in yeast |
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Northern and Southern blots |
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RT-PCR analysis |
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Please do not hesitate to contact us for your molecular biology needs. We are confident that we can provide answers to the problems you may face in this area, and that we will be able to perform the required service for you! |
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